Human Luteinizing hormone (LH) is a glycoprotein hormone secreted by the anterior pituitary. This hormone is composed of α and β subunits. The amino acid sequence of α-LH is essentially identical to that of other hormones including follicle stimulating hormone (FSH), thyroid stimulating hormone (TSH) and human Chorionic Gonadotropin (HCG). It is β subunit of LH that confers the biological and immunochemical specificity of the hormone. LH and FSH together with other steroid hormones are known to play important roles in regulating the ovulation and ovarian functions during the menstrual cycle. Maturation of an ovarian follicle and its oocyte begins during the end of the preceding menstrual cycle. In response to FSH released by the pituitary, the follicle undergoes rapid growth. As follicles develop, estradiol secretion begins to rise slowly and is followed by a rapid increase. This increase of estradiol level is gene.